ABSTRACT
<p><b>OBJECTIVE</b>To develop a RP-HPLC method for determination of cinnamic acid in rat plasma.</p><p><b>METHOD</b>The plasma samples were acidified with acetic acid and extracted with chloroform. Cinnamic acid was separated on a Kromasil C18 column (250 mm x 4.6 mm, 5 microm) eluted with a mobile phase of methanol-acetonitrile-water-glacial acetic acid (25:20:55:0.3) at a flow rate of 1.0 mL x min(-1) and room temperature with UV detection at 278 nm, carbamazepine as internal standard.</p><p><b>RESULT</b>The standard curve was linear over the range of 4.0 to approximately 400 ng x mL (-1) r = 0..99 9. The LOQ was 4.0 ng x mL(-1), the mean extraction recovery of the spiked samples at low, middle and high levels was 86.4%, while the mean method recovery was 100.3%. The RSD of intra-day and inter-day were both less than 6.0%.</p><p><b>CONCLUSION</b>The method was sensitive, specific, accurate and precise, which was used to study the pharmacokinetic profile of cinnamic acid in rat plasma after oral administration of the Subing orally disintegrating tablets.</p>
Subject(s)
Animals , Male , Rats , Administration, Oral , Area Under Curve , Chromatography, High Pressure Liquid , Methods , Cinnamates , Blood , Pharmacokinetics , Drug Combinations , Drugs, Chinese Herbal , Pharmacokinetics , Liquidambar , Chemistry , Plants, Medicinal , Chemistry , Rats, Wistar , Reproducibility of Results , TabletsABSTRACT
<p><b>OBJECTIVE</b>The fluorescence property of sophoramine was studied and a spectrofluorimetric method was established to determine the sophoramine content.</p><p><b>METHOD</b>In 20% ethanol solution, with excitation wavelength at 394 nm and emission wavelength at 467 nm, the fluorescence intensity of sophoramine can be detected by the fluorophotometer.</p><p><b>RESULT</b>Sophoramine content can be determined with external standard method by fluorophotometer. The linear relationship between fluorescence intensity and concentration is kept in the range of 10-200 microg x mL(-1). The regression equation is Int = 1.137C + 3.875. The recovery rate is 98%-102%.</p><p><b>CONCLUSION</b>Utilizing the fluorescence character of sophoramine, its content can be determined fast and sensitively. The analysis is not interfered by the existing matrine and oxymatrine. The method has high selectivity and the results is satisfying.</p>